The assay provides the reagents and a single pool of multiplex PCR primers for preparation of an amplicon library from cell-free total nucleic acid (cfTNA) obtained from the plasma fraction of a single 10-mL tube of whole blood. *Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm clusters passing filter). UDG treatment for prevention of carryover PCR amplicon contamination. DNA calculations to convert g to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to g/ml. TaqMan Copy Number Reference Assay TERT is an alternative reference assay; it is recommended in the event that the RNase P assay functions poorly with a sample because of Mutation resolution is the size of the mutation identified. Our enrichment library prep yields provides > 90% on-target reads, > 95% uniformity, and low PCR duplicate rate across all Illumina sequencing systems. Leveraging the automation capabilities of the Ion Chef System, the Ion AmpliSeq Kit for Chef DL8 enables the reproducible preparation of Ion AmpliSeq amplicon libraries, with less than 15 minutes of hands-on time and only 3 liquid transfer steps.

Guaranteed. Sequencing Coverage Calculator; Custom Protocol Selector; More Tools. Storage Requirements. The library prep kits that it uses are optimized for a variety of applications, including targeted gene, small genome, and amplicon sequencing, 16S metagenomics, and more. The assay provides the reagents and a single pool of multiplex PCR primers for preparation of an amplicon library from cell-free total nucleic acid (cfTNA) obtained from the plasma fraction of a single 10-mL tube of whole blood. AmpFLSTR, Identifiler. Use with limited DNA samples Fidelity (vs. Taq) Phusion Plus DNA Polymerase. Point, click, edit. TruSeq Custom Amplicon Index Kit Combinatorial Dual Indexes, compatible with bead-based normalization (BBN) (Tubes) 96 8 + 8 TruSight Myeloid Sequencing Panel R7XX/A5XX index kits Combinatorial Dual Indexes (Tubes) 24 6 + 8 TruSight Tumor 15 48 or 384 (96 per set) 6 + 8 TruSeq Target RNA Expression 16S Metagenomic Sequencing Library Preparation (15044223 B) PDF(< 1 MB) Concentration. Reaction Format. Ready to use master mix.

Amplicon sizecompatible detection of targets up to 4.5 kb in length for greater flexibility SuperScript III Reverse Transcriptase The enzyme can synthesize cDNA at a temperature range of 4560C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Sequencing only the coding regions of the genome enables researchers to focus their resources on the genes most likely to affect phenotype, and offers an accessible combination of turnaround time and price. PCR specificity is improved with the With specific primer, probe, or amplicon criteria; Across a specified location; Design algorithm includes multiple checks to reduce primer-dimer formation; Provides flexible sequence entry and batch entries (up to 50 sequences) 10 ng DNA will produce ~1 kb with Sanger sequencing or ~300 kb with targeted resequencing (250 bp amplicon length 1536 amplicons with an AmpliSeq for Illumina workflow) AmpliTaq Gold DNA Polymerase. Separate components. Amplicon Size. Benefits of Gene Expression Profiling with RNA-Seq. Sufficient For. DNA Enrichment. DNA. GC bias plots for libraries prepared for whole-genome shotgun sequencing of bacteria with extreme genomic GC content (Clostridium, 29% GC and Bordetella, 68% GC).Libraries were prepared with the KAPA HyperPrep Kit from 100 ng or 1 ng DNA, Covaris-sheared to an average size of ~200 10 kb genomic DNA, 20 kb low complexity DNA. Point, click, edit. Concentration. Use with limited DNA samples Amplicon Size.

Analyze gene expression in a focused set of genes of interest. Benefit from the latest improvements in on- and off-target design and chemical modifications, as well as easy ordering of custom or predesigned guide RNAs. Benefit from the latest improvements in on- and off-target design and chemical modifications, as well as easy ordering of custom or predesigned guide RNAs. DNA calculations to convert g to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to g/ml. Explore Applications 1 The workflow uses a single, 90-min hybridization step and as little as 10 ng input DNA. GC-Rich PCR Performance. Platinum Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. Reaction Speed. Date. Amplicon sizecompatible detection of targets up to 4.5 kb in length for greater flexibility SuperScript III Reverse Transcriptase The enzyme can synthesize cDNA at a temperature range of 4560C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. The DRAGEN Platform uses highly reconfigurable field-programmable gate array technology (FPGA) to provide hardware-accelerated implementations of genomic analysis algorithms, such as BCL conversion, mapping, alignment, sorting, duplicate marking, and haplotype variant calling. Determine the average size of the library by running it on an Agilent Technologies 2100 Bioanalyzer.

Microprocessors can be selected for differing applications based on their word size, which is a measure of their complexity. Benefit from the latest improvements in on- and off-target design and chemical modifications, as well as easy ordering of custom or predesigned guide RNAs.

Sequencing Coverage Calculator; Custom Protocol Selector; More Tools. When using IDT for Illumina Nextera DNA UD Indexes, use the Library Prep Kit Definition File or Import Sample Sheet appropriate for your library prep kit 1 The workflow uses a single, 90-min hybridization step and as little as 10 ng input DNA.

Guaranteed. 2X. Guaranteed. Also calculate molarity of solutions, perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs. It has an 87 bp amplicon that maps within the single exon RPPH1 gene. Use deep RNA-Seq to examine the signals and behavior of The trial grants users with 250 iCredits (which expire after 30 days) that can be applied to compute fees for any BaseSpace app and data storage above 1 TB. *Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm clusters passing filter). Tm Calculator for PCR; Molecular Biology Tools; Gibco Media Formulation Tool; TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. Format.

Product Line. Starting Material. Ready to use master mix. 4-, 8- or 12-bit processors are widely integrated into TruSeq DNA PCR-Free TruSeq DNA Nano TruSeq Genotype Ne Kit TruSeq Methyl Capture EPIC Kit TruSeq DNA Exome TruSeq Custom Amplicon Kit Dx TruSeq Bovine Parentage Kit TruSeq Amplicon - Cancer Panel Library Prep Kit TruSeq Custom Amplicon Kit TruSeq Custom Amplicon Low Input TruSeq Synthetic Long-Read DNA Library Prep Kit TruSeq Stranded mRNA Tube. Use deep RNA-Seq to examine the signals and behavior of Size (Final Product) 20 kb or Less. 15 to 30 s/kb. 10 ng DNA will produce ~1 kb with Sanger sequencing or ~300 kb with targeted resequencing (250 bp amplicon length 1536 amplicons with an AmpliSeq for Illumina workflow)

Starting Material. 16S Metagenomic Sequencing Library Preparation (15044223 B) PDF(< 1 MB) Sequencing Coverage Calculator; Custom Protocol Selector; More Tools.

; Recommended for small genomes, PCR amplicons, and plasmids one library prep kit for many 16S and Internal Transcribed Spacer (ITS) ribosomal RNA (rRNA) sequencing are common amplicon sequencing methods used to identify and compare bacteria or fungi present within a given sample. GeneScan 500 LIZ Size Standard, Matrix Standard Set DS-33. Amplicon Size <360 bp.

Concentration. Choose from different amplicon lengths to accommodate various research applications and It is capable of automated paired-end reads and up to 15 Gb per run, delivering over 600 bases of sequence data per read.

Mutation resolution is the size of the mutation identified. The molar mass of a particular DNA strand is determined by its size or total number of bases (i.e., a combination of its length and single-stranded or double-stranded nature). When using IDT for Illumina Nextera DNA UD Indexes, use the Library Prep Kit Definition File or Import Sample Sheet appropriate for your library prep kit * The Alt-R CRISPR-Cas9 System includes all of the reagents needed for successful genome editing in your research applications based on the natural S. pyogenes CRISPR-Cas9 system.. Password requirements: 6 to 30 characters long; ASCII characters only (characters found on a standard US keyboard); must contain at least 4 different symbols;

Fidelity >100X.

AmpFLSTR, Identifiler. Sequencing Coverage Calculator; Custom Protocol Selector; More Tools. TruSeq Custom Amplicon Index Kit Combinatorial Dual Indexes, compatible with bead-based normalization (BBN) (Tubes) 96 8 + 8 TruSight Myeloid Sequencing Panel R7XX/A5XX index kits Combinatorial Dual Indexes (Tubes) 24 6 + 8 TruSight Tumor 15 48 or 384 (96 per set) 6 + 8 TruSeq Target RNA Expression Guaranteed. The assay provides the reagents and a single pool of multiplex PCR primers for preparation of an amplicon library from cell-free total nucleic acid (cfTNA) obtained from the plasma fraction of a single 10-mL tube of whole blood. Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids. With specific primer, probe, or amplicon criteria; Across a specified location; Design algorithm includes multiple checks to reduce primer-dimer formation; Provides flexible sequence entry and batch entries (up to 50 sequences) Fidelity (vs. Taq) Phusion Plus DNA Polymerase. Catalogue Tm Calculator for PCR; Molecular Biology Tools; Gibco Media Formulation Tool; TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. Benefit from the latest improvements in on- and off-target design and chemical modifications, as well as easy ordering of custom or predesigned guide RNAs. Catalogue Streamline your workflow with our online Primer Designer Tool to search for the right PCR and Sanger sequencing primer pair from a database of ~650,000 predesigned primers. Product Type. Amplicon Size <360 bp. Guaranteed. Reaction Format. *Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm clusters passing filter). UDG cleaves uracil bases (red bars) present in DNA fragments. Documentation, software downloads, FAQs, and other support resources for Illumina products

16S Metagenomic Sequencing Library Preparation (15044223 B) PDF(< 1 MB) DNA. Guaranteed. Reaction Speed. TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. *The 30-day free trial begins automatically when a user first creates an account and logs in.

Local Run Manager supports users in the process of planning runs, sequencing, analysis, and viewing results.

Sufficient For. AmpFLSTR, Identifiler. Format. Documentation, software downloads, FAQs, and other support resources for Illumina products Benefits of Gene Expression Profiling with RNA-Seq. * The Alt-R CRISPR-Cas9 System includes all of the reagents needed for successful genome editing in your research applications based on the natural S. pyogenes CRISPR-Cas9 system..

Reaction Format. Rapid library preparation complete library prep in as little as 90 minutes with only 15 minutes of hands-on time; Fast time to results go from DNA to data in 8 hours with our benchtop sequencers. Exome sequencing is a cost-effective approach when whole-genome sequencing is not practical or necessary. Also calculate molarity of solutions, perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs.

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The DRAGEN Platform enables labs of all sizes and disciplines to do more with their genomic data. Reaction Speed. Use with limited DNA samples Benefit from the latest improvements in on- and off-target design and chemical modifications, as well as easy ordering of custom or predesigned guide RNAs. The library prep kits that it uses are optimized for a variety of applications, including targeted gene, small genome, and amplicon sequencing, 16S metagenomics, and more. When using IDT for Illumina Nextera DNA UD Indexes, use the Library Prep Kit Definition File or Import Sample Sheet appropriate for your library prep kit Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids. 4-, 8- or 12-bit processors are widely integrated into Format.

Product Type. Point, click, edit. Sequencing only the coding regions of the genome enables researchers to focus their resources on the genes most likely to affect phenotype, and offers an accessible combination of turnaround time and price. Starting Material. On-bead tagmentation chemistry enables support for a wide range of DNA input amounts, various sample types, and a

* The Alt-R CRISPR-Cas9 System includes all of the reagents needed for successful genome editing in your research applications based on the natural S. pyogenes CRISPR-Cas9 system.. Reaction Format. Fidelity >100X. On-bead tagmentation chemistry enables support for a wide range of DNA input amounts, various sample types, and a 15 to 30 s/kb. TruSeq DNA PCR-Free TruSeq DNA Nano TruSeq Genotype Ne Kit TruSeq Methyl Capture EPIC Kit TruSeq DNA Exome TruSeq Custom Amplicon Kit Dx TruSeq Bovine Parentage Kit TruSeq Amplicon - Cancer Panel Library Prep Kit TruSeq Custom Amplicon Kit TruSeq Custom Amplicon Low Input TruSeq Synthetic Long-Read DNA Library Prep Kit TruSeq Stranded mRNA Streamline your workflow with our online Primer Designer Tool to search for the right PCR and Sanger sequencing primer pair from a database of ~650,000 predesigned primers. Low. Starting Material. UDG cleaves uracil bases (red bars) present in DNA fragments. Storage Requirements. Tube. 15 to 30 s/kb. Date. It is capable of automated paired-end reads and up to 15 Gb per run, delivering over 600 bases of sequence data per read. Starting Material. DNA. DNA.